Molecular Biology

Alkaline Lysis Plasmid Maxi-Prep (Download)

 

AAV Preparation (Download)

 

BES Transfection (neurons) (Download)

 

Calcium Phophate Transfection (HEK293T) (Download)

DNA Purification Buffers

Genotyping PCR (Download 1, 2)

Gibson Assembly

HEK Cell Binding Assay (Download)

Live surface labeling of Neurons (Download)

Neuron Culture Immunocytochemistry (Download)

Purification of High-Fidelity Proof Reading Polymerase (Download)

TAQ Purification (Download)

6x DNA Loading Dye (Download)

Qiagen Buffer recipes (Download)

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Neuron Culture Protocols

Neuron Media (Download)

Isolation Protocol (Download)

 

Electrophysiology

ACSF Recipe (Download)

 

Cesium Methanesulfonate Internal (Download)

Non-Oxygenated HEPES ACSF (Download)

Potassium Gluconate Internal (Download)

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Sucrose Cutting Solution (Download)