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    Aoto Lab | University of Colorado, Denver | Department of Pharmacology | 12800 E. 19th Ave. | Mail Stop: 8303 | Aurora, CO 80045
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    Aoto Lab 2016

    Updated 10/12/19

    Molecular Biology
    Alkaline Lysis Plasmid Maxi-Prep (Download)
     
    AAV Preparation (Download)
     
    BES Transfection (neurons) (Download)
     
    Calcium Phophate Transfection (HEK293T) (Download)
    DNA Purification Buffers
    Genotyping PCR (Download 1, 2)
    Gibson Assembly
    HEK Cell Binding Assay (Download)
    Live surface labeling of Neurons (Download)
    Neuron Culture Immunocytochemistry (Download)
    Purification of High-Fidelity Proof Reading Polymerase (Download)
    TAQ Purification (Download)
    6x DNA Loading Dye (Download)
    Qiagen Buffer recipes (Download)
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    Neuron Culture Protocols
    Neuron Media (Download)
    Isolation Protocol (Download)
     
    Electrophysiology
    ACSF Recipe (Download)
     
    Cesium Methanesulfonate Internal (Download)
    Non-Oxygenated HEPES ACSF (Download)
    Potassium Gluconate Internal (Download)
    ​
    Sucrose Cutting Solution (Download)